Transfer Rna : Molecular Structure , Sequence , and Properties 0934

CONTENTS INTRODUCTION ............................................................................................................ 806 THE MULTIPLE BIOLOGICAL FUNCTIONS F tRNA ................................................ 807 tRNA Cycle in Protein Synthesis .............................................................................. 807 tRNA and the Regulation of Enzyme Synthesis ...................................................... 807 Aminoacyl-tRNA Transferases .................................................................................. 808 tRNA Participation in Polynucleotide Synthesis ........................................................ 808 tRNA as an Enzyme Inhibitor .................................................................................. 808 tRNA Changes in Cells .............................................................................................. 809 NEWER METHODS FOR PURIFICATION AND SEQUENCE ANALYSIS OF tRNA ...... 809 Purification f tRNAs ................................................................................................ 810 Sequence Analysis of tRNA ...................................................................................... 812 GENERAL FEATURES OF tRNA SEQUENCES .............................................................. 813 Generalized Secondary Structure for tRNAs ............................................................ 815 Invariant and Semi-invariant Nucleotides in tRNAs ................................................ 817 Unique Features in Initiator tRNA Sequences .......................................................... 818 MOLECULAR STRUCTURE OF NUCLEIC ACID COMPONENTS AND DOUBLE HELICAL NUCLEIC ACIDS ............................................................................ 819 CRYSTALLIZATION OF tRNA ........................................................................................ 821 High Resolution Crystals of Yeast RNel TM . ............................................................. 823 Solution of X-ray Diffraction Patterns Using Heavy-Atom Derivatives .................. 823 SOLUTION OF THE YEAST tRNATM STRUCTURE BY X-RAY DIFFRACTION ............ 825 Folding of the Polynucleotid, e Chain at 4-.~ Resolution--1973 ................................ 825 Tertiary Interactions at 3-A Resolution--1974, ........................................................ 827 Tertiary Interactions and Coordinates at 2.5-A Resolutions--1975 .......................... 828 THREE-DIMENSIONAL STRUCTURE OF YEAST tRNA~ ............................................ 829 Aeceptor Stem ............................................................................................................ 829 T~C Stem and Loop ................................................................................................ 830 D Stem and Loop ...................................................................................................... 835 Anticodon Stem and Loop ........................................................................................ 837